产品具体信息请参见:
http://www.yrgene.com/goods-123245.html
货号:VPI0012
载体名称:pcDNA3.1/V5-His C
出品公司:Invitrogen
载体用途:哺乳动物表达载体
价格:900元
规格:一管质粒,5ug。附送一管甘油菌,500ul。
到货周期:现货
原核抗性:Ampicillin(氨苄青霉素)
真核筛选标记:Neomycin / G418
启动子:CMV promter
标签:C端带V5标签及His标签
表达水平:高
5 测序引物:CMV-f
3 测序引物:BGH-r
载体拷贝数:高
载体描述:
pcDNA ™ 3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA ™ 3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells.
The vectors contain the following elements:
(1)Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide
(2)Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
(3)Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7)
The control plasmid, pcDNA ™ 3.1/V5-His/lacZ, contains a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. pcDNA ™ 3.1/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.
产品说明书及载体序列:
请访问以下页面下载【pcDNA3.1/V5-His C】产品说明书及载体序列:
http://www.yrgene.com/goods-123245.html
质粒图谱: