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赢润生物 pcDNA3.1/V5-His C 载体 质粒
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产品: 浏览次数:336赢润生物 pcDNA3.1/V5-His C 载体 质粒 
品牌: YRGene
单价: 900.00元/
最小起订量:
供货总量:
发货期限: 自买家付款之日起 3 天内发货
有效期至: 长期有效
最后更新: 2018-04-09 09:57
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详细信息

产品具体信息请参见:

http://www.yrgene.com/goods-123245.html

货号:VPI0012    
载体名称:pcDNA3.1/V5-His C    
出品公司:Invitrogen
载体用途:哺乳动物表达载体    
价格:900元
规格:一管质粒,5ug。附送一管甘油菌,500ul。    
到货周期:现货

原核抗性:Ampicillin(氨苄青霉素)

真核筛选标记:Neomycin / G418

启动子:CMV promter

标签:C端带V5标签及His标签

表达水平:

5 测序引物:CMV-f

3 测序引物:BGH-r

载体拷贝数:

载体描述:

pcDNA ™ 3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA ™ 3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells.

 

The vectors contain the following elements:
(1)Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide
(2)Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
(3)Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7)

The control plasmid, pcDNA ™ 3.1/V5-His/lacZ, contains a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. pcDNA ™ 3.1/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

产品说明书及载体序列:
请访问以下页面下载pcDNA3.1/V5-His C产品说明书及载体序列:
http://www.yrgene.com/goods-123245.html

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